Journal: Theranostics

Article Title: Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes

doi: 10.7150/thno.80801

Figure Lengend Snippet: Antibodies used for immunostaining

Article Snippet: Rabbit anti-myosin light chain ventricular isoform , 1:600 , 10906-1-AP , Proteintech , Goat anti-rabbit IgG-FITC , 1:800 , A32790 , Thermo Fisher.

Techniques:

Journal: Theranostics

Article Title: Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes

doi: 10.7150/thno.80801

Figure Lengend Snippet: Antibodies used for fluorescence activated cell sorting

Article Snippet: Rabbit anti-myosin light chain ventricular isoform , 1:600 , 10906-1-AP , Proteintech , Goat anti-rabbit IgG-FITC , 1:800 , A32790 , Thermo Fisher.

Techniques: Fluorescence

Journal: Theranostics

Article Title: Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes

doi: 10.7150/thno.80801

Figure Lengend Snippet: Antibodies used in Western Blot analysis

Article Snippet: Rabbit anti-myosin light chain ventricular isoform , 1:600 , 10906-1-AP , Proteintech , Goat anti-rabbit IgG-FITC , 1:800 , A32790 , Thermo Fisher.

Techniques: Western Blot

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 4. Effect of yak milk on immunohistochemistry of kidney in mice. (A) Images of mouse kidney tissue cross sections immunostained for TRPV5 and calbindin-D28k (scale bar = 100 μm). (B) Relative mean density of TRPV5 in mouse kidney. (C) Relative mean density of calbindin-D28k in mouse kidney. Mean density = (integrated optical density [IOD] sum)/area. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Immunohistochemistry

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 5. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k in mouse kidney. (A) Western blot showing the expression of TRPV5 and calbindin-D28k in mouse kidney. (B) Quantification of TRPV5 expression from the Western blots. (C) Quantification of calbindin-D28k expression from the Western blots. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with dif- ferent lowercase letters (a–c) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing, Western Blot

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 6. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k mRNA in mouse kidney. (A) Relative expression of TRPV5 mRNA in mouse kidney. (B) Relative expression of calbindin-D28k mRNA in mouse kidney. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing

Journal: Pharmaceutical Biology

Article Title: Gastrodin attenuates angiotensin II-induced vascular contraction and MLCK/p-MLC 2 pathway activation

doi: 10.1080/13880209.2023.2207591

Figure Lengend Snippet: Expression of proteins involved in the MLCK/p-MLC 2 pathway in abdominal aorta tissues. Immunohistochemical (IHC) staining was used to detect protein levels of (A) phospho-myosin light chain 2 (p-MLC 2 ), (B) myosin light chain 2 (MLC 2 ), (C) angiotensin type 1 receptor (AT1R), (D) calmodulin (CaM) and (E) myosin light chain kinase (MLCK) in abdominal aorta tissues. Images were taken at a magnification of ×400. All values are represented as mean ± SD; * p < 0.05 Ang II vs. control group; # p < 0.05 Ang II + gastrodin vs. Ang II group.

Article Snippet: Antibodies against MLCK (cat. no. 48846), p-MLC 2 (cat. no. 11114) and α-SMA (cat. no. 40482) were purchased from Signalway Antibody LLC (Greenbelt, MD).

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry

Journal: Pharmaceutical Biology

Article Title: Gastrodin attenuates angiotensin II-induced vascular contraction and MLCK/p-MLC 2 pathway activation

doi: 10.1080/13880209.2023.2207591

Figure Lengend Snippet: The effects of gastrodin on the MLCK/p-MLC 2 pathway in Ang II-stimulated VSMCs. Western blotting was used to determine protein levels of (A) p-MLC 2 , (B) AT1R, (C) CaM and (D) MLCK in Ang II-stimulated VSMCs pretreated with gastrodin (50, 100 or 200 μM) for 24 h. All values are represented as mean ± SD; * p < 0.05 Ang II vs. control group; # p < 0.05 vs. Ang II group.

Article Snippet: Antibodies against MLCK (cat. no. 48846), p-MLC 2 (cat. no. 11114) and α-SMA (cat. no. 40482) were purchased from Signalway Antibody LLC (Greenbelt, MD).

Techniques: Western Blot